Workflow Type: Common Workflow Language
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Stable
Amplicon analysis workflow using NG-Tax
Steps:
- Quality control on the reads
- Execute NGTax for ASV detection and classification
For more information about NG-Tax 2.0 have a look at https://doi.org/10.3389/fgene.2019.01366
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Inputs
ID | Name | Description | Type |
---|---|---|---|
forward_reads | forward reads | forward sequence file locally |
|
reverse_reads | reverse reads | reverse sequence file locally |
|
forward_primer | Forward primer | Forward primer used |
|
reverse_primer | Reverse primer | Reverse primer used |
|
reference_db | Reference database | Reference database used in FASTA format |
|
rev_read_len | Reverse read length | Read length of the reverse read |
|
for_read_len | Reverse read length | Read length of the reverse read |
|
sample | Sample name | Name of the sample being analysed |
|
Steps
ID | Name | Description |
---|---|---|
fastqc | n/a | n/a |
reads_to_folder | n/a | n/a |
ngtax | n/a | n/a |
fastqc_files_to_folder | n/a | n/a |
ngtax_files_to_folder | n/a | n/a |
Outputs
ID | Name | Description | Type |
---|---|---|---|
files_to_folder_fastqc | n/a | n/a |
|
files_to_folder_ngtax | n/a | n/a |
|
Version History
Version 7 (latest) Created 28th Oct 2020 at 11:07 by Jasper Koehorst
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Creators and Submitter
Creators
Additional credit
Jesse van Dam, Peter Schaap,
Submitter
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Activity
Views: 10216 Downloads: 349
Created: 23rd Jul 2020 at 10:59
Last updated: 8th Jun 2021 at 08:11
Attributions
None