Workflow 2D relion (tilted experiments variation) Version 1
Download RO-CrateAutomated image processing from movies to 2D classes. Includes CTF estimation, quality filters, box size estimation, training a data-specific picking model, and three 2D classification jobs (25k, 50k, and 100k particles) using Relion.
Adapting to Tilted Samples
To process tilted experiments, use ”tilted” templates. These are modified workflows tailored to handle high drift and lower resolution commonly observed in tilted samples. Key adjustments include:
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Motion Correction Filters: Max per-frame shift = 30; Global drift = 120.
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CTF Consensus Filters: Adapted to 6.5 Å and 8.5 Å resolution cutoffs.
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Defocus Sampler: Trains with 500 images to select 25 diverse examples, ensuring a generalizable picking model.
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Version 1 (earliest) Created 24th Jun 2025 at 11:28 by Daniel Marchan
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Created: 24th Jun 2025 at 11:28
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This document provides guidance for understanding and implementing the set of workflows included in this collection. It outlines best practices and recommendations to ensure successful setup and execution of automated and real-time image processing pipelines.
Creator: Daniel Marchan
Submitter: Daniel Marchan
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A collection of image processing templates designed to support data collection and automated processing for Single Particle Analysis (SPA) in cryo-electron microscopy. The workflows range from simple pipelines—including movie alignment, CTF estimation, and quality assessment—to fully automated 2D and 3D processing. Please select the workflow that best suits your needs.
