Research Object Crate for Workflow 2D relion (tilted experiments variation)

Original URL: https://workflowhub.eu/workflows/1752/ro_crate?version=1

Automated image processing from movies to 2D classes. Includes CTF estimation, quality filters, box size estimation, training a data-specific picking model, and three 2D classification jobs (25k, 50k, and 100k particles) using Relion. **Adapting to Tilted Samples** To process tilted experiments, use ”tilted” templates. These are modified workflows tailored to handle high drift and lower resolution commonly observed in tilted samples. Key adjustments include: * **Motion Correction Filters**: Max per-frame shift = 30; Global drift = 120. * **CTF Consensus Filters**: Adapted to 6.5 Å and 8.5 Å resolution cutoffs. * **Defocus Sampler**: Trains with 500 images to select 25 diverse examples, ensuring a generalizable picking model.

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Contents

Main Workflow: Workflow 2D relion (tilted experiments variation)
Size: 28034 bytes
Main Workflow Diagram: 2D_Relion.png
Size: 125462 bytes