Workflows
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Type: Nextflow
Creators: Gisela Gabernet, Simon Heumos, Alexander Peltzer
Submitter: WorkflowHub Bot
CLIP-seq Workflow
A Nextflow workflow for end-to-end processing of CLIP-seq data, supporting multiple CLIP protocols.
Overview
Starting from raw FASTQ files (or un-demultiplexed iCLIP data), the workflow processes reads through quality control, adapter trimming, rRNA removal, genome alignment, and UMI deduplication, then runs shoji to extract crosslink sites and produce per-sample and combined count matrices ready for differential binding analysis (see ...
Petrisnake: A secondary analysis pipeline for PETRI-seq data.
This is a Snakemake pipeline for the secondary computational analysis of single cell RNA-seq data from the PETRI-seq protocol (https://www.nature.com/articles/s41564-020-0729-6 and https://www.nature.com/articles/s41586-024-08124-2), this is: From the input FASTQ files, this workflow constructs a gene count table showing the expression of each gene in each cell. Petrisnake is available on WorkflowHub (https://workflowhub.eu/workflows/2081). ...
MPXV (Mpox) Phylogenetic Analysis with Squirrel
Description
Galaxy workflow to perform MPXV phylogenetic reconstruction using the Squirrel (Some QUIck Reconstruction to Resolve Evolutionary Links) Galaxy tools, Squirrel QC and Squirrel Phylo. The workflow also performs masking (using the squirrel tool) of SNPs.
Galaxy Squirrel tools:
Squirrel QC: The quality control (QC) mode of Squirrel that can run QC on the alignment and flag certain sites ...
Viral Amplicon Analysis Pipeline for ONT Data
Description
Galaxy workflow for processing viral amplicon datasets, such as MPXV (Mpox), sequenced using the Oxford Nanopore Technologies (ONT) platform. The workflow uses the latest Fieldbioinformatics Artic Minion pipeline that uses the clair3 variant caller.
The ARTIC minion is a pipeline for working with viral nanopore sequencing data, generated from tiling amplicon schemes. It is designed ...
Automated screening monitoring workflow designed to provide real-time feedback to the microscope during data acquisition. The pipeline processes movies on-the-fly, performing motion correction, maxshift analysis, CTF estimation and consensus validation, as well as micrograph quality assessment through AI-based categorization. The workflow also includes particle picking, automatic box size estimation, particle extraction, streaming 2D classification, and 2D class quality assessment. Results are ...
This workflow performs a basic Virtual Drug Screening Pipeline. THe workflows uses the following protocols: 1.a) Import receptor: Imports the atomic structure with PDB code 4ERF. 1.b) Import small molecules: Imports a set of 4 molecules from local files. 2.a) Receptor preparation: selects A chain and cleans the structure from waters and heteroatoms 2.b) RDKit Molecules preparation: adds hydrogens and prepares the molecules for docking 3.a) Find pockets: uses P2Rank to predict the most promising ...
Tests