Workflows
What is a Workflow?Filters
Detailed description of your COMPSs application
Workflow for quality assessment and taxonomic classification of amplicon long read sequences. In addition files are exported to their respective subfolders for easier data management in a later stage.
Inputs are expected to be basecalled fastq files
Steps:
- NanoPlot read quality control, before and after filtering
- fastplong read quality and length filtering
- Emu abundance; species-level taxonomic abundance for full-length 16S read
Workflow for long read quality control, contamination filtering, assembly, variant calling and annotation.
- Preprocessing of reference file
- LongReadSum before and after filtering (read quality control)
- Filtlong filter on quality and length
- Flye assembly
- Minimap2 mapping of reads and assembly
- Clair3 variant calling of reads
- Freebayes variant calling of assembly
- Optional Bakta annotation of genomes with no reference
- SnpEff building or downloading of a database
- SnpEff functional ...
Workflow for preprocessing the reference file. Downloads the GenBank file from NCBI if not provided, concatenates plasmid GenBank file(s) with each other and the reference file, extracts GFF3 from the (merged) reference.
This workflow on WorkflowHub: https://workflowhub.eu/workflows/1818
All tool CWL files and other workflows can be found here: Tools: https://git.wur.nl/ssb/automated-data-analysis/cwl/-/tree/main/tools Workflows: https://git.wur.nl/ssb/automated-data-analysis/cwl/-/tree/main/workflows ...
Citation
If you use this workflow in your research, please cite it. Use the "Cite this
repository" button on the GitHub repository page (generated from
CITATION.cff), or cite the archived release on
...
This is the workflow for the Batch Correction and Integration tutorial if you use Seurat.
Associated Tutorial
This workflows is part of the tutorial Batch Correction and Integration with Seurat or Scanpy, available in the GTN
Features
- Includes Galaxy Workflow Tests ...
Workflow for GTN tutorial on single-cell batch correction, using the Scanpy pipeline with Harmony.
Associated Tutorial
This workflows is part of the tutorial Batch Correction and Integration with Seurat or Scanpy, available in the GTN
Features
- Includes Galaxy Workflow Tests ...
Automated image processing from movies to 2D classification. Includes quality and curator micrgographs protocols as Dose analysis, maxshift, tilt analysis, categorize micrographs, ctf consensus, also include particle curator as Remove duplicates and Deep micrograph cleaner. It also include a support branch to calculate the Box Size and train a model to pick the particles. The list of plugins required are: pwem, xmipp3, motioncorr, miffi, cistem, emfacilities, sphire, gautomatch, relion, repic ...
Introduction
https://github.com/rodtheo/nf-core-assemblyeval
Assemblyeval accepts genome assemblies (FASTA), paired-end Illumina reads, and long reads (ONT or PacBio) via a YAML samplesheet, optionally cleaning them of contamination before evaluation. The pipeline systematically assesses contiguity (QUAST), completeness (COMPLEASM/BUSCO, Merfin), and correctness (ALE, REAPR, CRAQ) — combining read-alignment-based and k-mer-based evidence ...