Workflows
What is a Workflow?Filters
The bioinformatic workflow presented here enables the analysis of RNA sequencing data obtained from human reproductive tissues in unexplained recurrent pregnancy loss (uRPL) research. This pipeline requires a sample sheet containing the sample information (example_input_data.csv) and gene expression matrices generated using the Salmon tool in the nf-core/rnaseq bioinformatics pipeline (example_count_data.csv). For more information on how to use the nf-core/rnaseq pipeline including the required ...
Type: Nextflow
Creators: Jasmin Frangenberg, Anan Ibrahim, James A. Fellows Yates
Submitter: WorkflowHub Bot
WorkflowHub Knowledge Graph
A tool to generate a knowledge graph from a source of RO Crates. By default, this tool sources and generates an RDF graph of crates from WorkflowHub.
Type: Snakemake
Creators: Alexander Hambley, Oliver Woolland, Eli Chadwick, Volodymyr Savchenko, José Mª Fernández, Stian Soiland-Reyes
Submitter: Eli Chadwick
Workflow for Metagenomics binning from assembly.
Minimal inputs are: Identifier, assembly (fasta) and an associated sorted BAM file
Summary
- MetaBAT2 (binning)
- MaxBin2 (binning)
- SemiBin2 (binning)
- Binette (bin merging)
- EukRep (eukaryotic classification)
- CheckM2 (bin completeness and contamination)
- BUSCO (bin completeness)
- GTDB-Tk (bin taxonomic classification)
- CoverM (bin abundances)
Including: Bin annotation (workflow: https://workflowhub.eu/workflows/1170):
- Bakta
...
Type: Common Workflow Language
Creators: Jasper Koehorst, Bart Nijsse
Submitters: Jasper Koehorst, Bart Nijsse
Type: Common Workflow Language
Creators: Ryo Mameda, Sora Yonezawa
Submitters: Sora Yonezawa, Ryo Mameda
Generate phased assembly based on PacBio HiFi reads and parental Illumina data for phasing. Part of the VGP workflow suite, it needs to be run after the Trio k-mer Profiling workflow VGP2. This workflow uses HiFiasm for contigging, and generates assembly statistics, BUSCO reports, Merqury plots, and the genome assembly contigs in fasta and GFA format.
MAGNETO is an automated snakemake workflow dedicated to MAG (Metagenome-Assembled Genomes) reconstruction from metagenomic data.
It includes a fully-automated coassembly step informed by optimal clustering of metagenomic distances, and implements complementary genome binning strategies, for improving MAG recovery.
Key Features
- Quality Control (QC): Automatically assesses the quality and the contamination of input reads, ensuring that low-quality data are filtered out to improve downstream ...
Type: Snakemake
Creators: Samuel Chaffron, Audrey Bihouee, Benjamin Churcheward, Maxime Millet, Guillaume Fertin, Hugo Lefeuvre
Submitter: Hugo Lefeuvre
This project is an analysis pipeline using Snakemake for RNAseq analysis in order to find differentially expressed genes. It has been widely tested on human RNA sequencing from an Illumina HiSeq but should work on most systems and many other species, provided the necessary resource files can be downloaded.
Description
This pipeline is set for paired-end data only from Illumina HiSeq output files.
The main steps of the pipeline are:
- optionnal cleaning data with ...
To perform Qualification, Nitrate Calibration, Validation of One Argo float or One Glider by using Neural Network and Climatology. Copy past before running and change calibration parametrization if necessary
Associated Tutorial
This workflows is part of the tutorial Nitrate DMQC for autonomous platforms such as Argo floats, available in the GTN ...
Soil Metagenome Pipeline
Soil Metagenome Pipeline is a modular, Nextflow DSL2 workflow for assembling, polishing, binning, annotating, and functionally characterizing complex soil metagenomes. It orchestrates state-of-the-art tools for long- and short-read metagenomics, generates high-quality MAGs, assigns taxonomy, and screens for biosynthetic gene clusters (BGCs).
What it does
- Assembles long-read metagenomes (e.g., ONT) with Flye and optionally polishes with Medaka and/or NextPolish using ...