CEITEC layer 1 workflow
Version 1

Workflow Type: Scipion

The second-level complexity workflow is one among a collection of workflows designed to address tasks up to CTF estimation. In addition to the functionalities provided by the layer 0 workflow, this workflow aims to enhance the quality of acquisition images using quality protocols.

Quality control protocols

  • Movie max shift: automatic reject those movies whose frames move more than a given threshold. 

  • Tilt analysis: quality score based in the Power Spectrum Density (astigmatism and tilt) 

  • CTF consensus: acts as a filter discarding micrographs based on their CTF (limit resolution, defocus, astigmatism, etc.).


  • More control of the acquisition quality

  • Reduce unnecessary processing time and storage

Version History

Version 2 (earliest) Created 4th Oct 2023 at 14:01 by Daniel Marchan

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Created: 4th Oct 2023 at 14:01

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