The second-level complexity workflow is one among a collection of workflows designed to address tasks up to CTF estimation. In addition to the functionalities provided by the layer 0 workflow, this workflow aims to enhance the quality of acquisition images using quality protocols.
Quality control protocols
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Movie max shift: automatic reject those movies whose frames move more than a given threshold.
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Tilt analysis: quality score based in the Power Spectrum Density (astigmatism and tilt)
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CTF consensus: acts as a filter discarding micrographs based on their CTF (limit resolution, defocus, astigmatism, etc.).
Advantages:
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More control of the acquisition quality
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Reduce unnecessary processing time and storage
Version History
Version 2 (latest) Created 10th Jul 2024 at 15:17 by Pablo Conesa
Testing versions. Actually same as version 1.
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Version 1 (earliest) Created 4th Oct 2023 at 14:01 by Daniel Marchan
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Views: 2455 Downloads: 715
Created: 4th Oct 2023 at 14:01
Last updated: 10th Jul 2024 at 15:21
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