Master of Pores 2
Version 1

Workflow Type: Nextflow

MoP2- DSL2 version of Master of Pores

Docker Build Status mop2-CI License: MIT Nextflow version Nextflow DSL2 Singularity version Docker version


Inspired by Metallica's Master Of Puppets


Please install nextflow and singularity or docker before.

Then download the repo:

git clone --depth 1 --recurse-submodules

You can use to download the version 3.4.5 of guppy or you can replace it with the version you prefer. Please consider that the support of VBZ compression of fast5 started with version 3.4.X.

cd MoP2; sh


You can replace -with-singularity with -with-docker if you want to use the docker engine.

cd mop_preprocess
nextflow run -with-singularity -bg -profile local > log


If you use this tool, please cite our papers:

"Nanopore Direct RNA Sequencing Data Processing and Analysis Using MasterOfPores" Cozzuto L, Delgado-Tejedor A, Hermoso Pulido T, Novoa EM, Ponomarenko J. N. Methods Mol Biol. 2023;2624:185-205. doi: 10.1007/978-1-0716-2962-8_13.

"MasterOfPores: A Workflow for the Analysis of Oxford Nanopore Direct RNA Sequencing Datasets" Luca Cozzuto, Huanle Liu, Leszek P. Pryszcz, Toni Hermoso Pulido, Anna Delgado-Tejedor, Julia Ponomarenko, Eva Maria Novoa. Front. Genet., 17 March 2020.


The documentation is available at

Version History

main @ e6eabb3 (earliest) Created 20th Feb 2023 at 15:48 by Luca Cozzuto


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Created: 20th Feb 2023 at 15:48

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