MoP2- DSL2 version of Master of Pores
Inspired by Metallica's Master Of Puppets
Please install nextflow and singularity or docker before.
Then download the repo:
git clone --depth 1 --recurse-submodules email@example.com:biocorecrg/MOP2.git
You can use INSTALL.sh to download the version 3.4.5 of guppy or you can replace it with the version you prefer. Please consider that the support of VBZ compression of fast5 started with version 3.4.X.
cd MoP2; sh INSTALL.sh
You can replace
-with-docker if you want to use the docker engine.
cd mop_preprocess nextflow run mop_preprocess.nf -with-singularity -bg -profile local > log
If you use this tool, please cite our papers:
"Nanopore Direct RNA Sequencing Data Processing and Analysis Using MasterOfPores" Cozzuto L, Delgado-Tejedor A, Hermoso Pulido T, Novoa EM, Ponomarenko J. N. Methods Mol Biol. 2023;2624:185-205. doi: 10.1007/978-1-0716-2962-8_13.
"MasterOfPores: A Workflow for the Analysis of Oxford Nanopore Direct RNA Sequencing Datasets" Luca Cozzuto, Huanle Liu, Leszek P. Pryszcz, Toni Hermoso Pulido, Anna Delgado-Tejedor, Julia Ponomarenko, Eva Maria Novoa. Front. Genet., 17 March 2020. https://doi.org/10.3389/fgene.2020.00211
The documentation is available at https://biocorecrg.github.io/MOP2/docs/
main @ e6eabb3 (earliest) Created 20th Feb 2023 at 15:48 by Luca Cozzuto
Created: 20th Feb 2023 at 15:48