Workflow 3D cryosparc (tilted experiments variation)

Version 1

Automated image processing from movies to 3D reconstruction. Includes CTF estimation, quality filters, box size estimation, picking model training, three 2D classification jobs (25k, 50k, and 100k particles), and 3D processing of the first 200k particles. Multiple 3D models are generated without symmetry, with automatic 2D and 3D class selection, using CryoSPARC.

Adapting to Tilted Samples

To process tilted experiments, use ”tilted” templates. These are modified workflows tailored to handle high drift and lower resolution commonly observed in tilted samples. Key adjustments include:

• Motion Correction Filters: Max per-frame shift = 30; Global drift = 120.

• CTF Consensus Filters: Adapted to 6.5 Å and 8.5 Å resolution cutoffs.

• Defocus Sampler: Trains with 500 images to select 25 diverse examples, ensuring a generalizable picking model.