vvv2_align_SE_minimap2_pacbio
Version 1

Workflow Type: Galaxy
Stable

Using:

  • vadr annotation (model to select)
  • vardict variant caller
  • coverage depth

Provides summarizing files:

  • png image of variant calling with annotations and coverage depths
  • tsv file with all information of significant variants only
  • vcf file with all information of significant variants only (to allow downstream NextStrain analyses)

Steps

ID Name Description
0 FASTA Width toolshed.g2.bx.psu.edu/repos/devteam/fasta_formatter/cshl_fasta_formatter/1.0.1+galaxy2
1 fastp toolshed.g2.bx.psu.edu/repos/iuc/fastp/fastp/0.23.2+galaxy0
2 VADR - Viral Annotation DefineR toolshed.g2.bx.psu.edu/repos/ftouzain/vadr/vadr/0.2.0
3 Map with minimap2 toolshed.g2.bx.psu.edu/repos/iuc/minimap2/minimap2/2.28+galaxy1
4 RmDup toolshed.g2.bx.psu.edu/repos/devteam/samtools_rmdup/samtools_rmdup/2.0.1
5 bcftools mpileup toolshed.g2.bx.psu.edu/repos/iuc/bcftools_mpileup/bcftools_mpileup/1.15.1+galaxy3
6 VarDict toolshed.g2.bx.psu.edu/repos/iuc/vardict_java/vardict_java/1.8.3+galaxy1
7 Samtools depth toolshed.g2.bx.psu.edu/repos/iuc/samtools_depth/samtools_depth/1.15.1+galaxy2
8 bcftools call toolshed.g2.bx.psu.edu/repos/iuc/bcftools_call/bcftools_call/1.15.1+galaxy3
9 vvv2_display - png image of variants - tsv file of variants info - vcf file of variants info toolshed.g2.bx.psu.edu/repos/ftouzain/vvv2_display/vvv2_display/0.2.5.0
10 IUPAC consensus toolshed.g2.bx.psu.edu/repos/iuc/bcftools_consensus/bcftools_consensus/1.15.1+galaxy3
11 majority consensus Gives consensus with major nucleotides only toolshed.g2.bx.psu.edu/repos/iuc/bcftools_consensus/bcftools_consensus/1.15.1+galaxy3

Outputs

ID Name Description Type
IUPAC_consensus #main/IUPAC_consensus n/a
  • File
Majority_consensus #main/Majority_consensus n/a
  • File
significant_variants_info #main/significant_variants_info n/a
  • File
significant_variants_info_for_NextStrain #main/significant_variants_info_for_NextStrain n/a
  • File
snp_image #main/snp_image n/a
  • File

Version History

Version 1 (earliest) Created 19th Jun 2025 at 11:27 by Fabrice Touzain

Initial commit


Open master f4cfc20
help Creators and Submitter
Creator
  • Fabrice Touzain
Additional credit

This study was founded by the French National Research Agency and by Santé publique France as part of the project "EMERGEN". Anses Ploufragan research was also supported by Agglomération de Saint-Brieuc, Département des Côtes d'Armor and Région Bretagne

Submitter
Activity

Views: 2171   Downloads: 290   Runs: 3

Created: 19th Jun 2025 at 11:27

Last updated: 3rd Apr 2026 at 15:19

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Total size: 542 KB
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