Workflow Type: Galaxy
Open
Stable
Using:
- vadr annotation (model to select)
- vardict variant caller
- coverage depth
Provides summarizing files:
- png image of variant calling with annotations and coverage depths
- tsv file with all information of significant variants only
- vcf file with all information of significant variants only (to allow downstream NextStrain analyses)
Steps
| ID | Name | Description |
|---|---|---|
| 0 | FASTA Width | toolshed.g2.bx.psu.edu/repos/devteam/fasta_formatter/cshl_fasta_formatter/1.0.1+galaxy2 |
| 1 | fastp | toolshed.g2.bx.psu.edu/repos/iuc/fastp/fastp/0.23.2+galaxy0 |
| 2 | VADR - Viral Annotation DefineR | toolshed.g2.bx.psu.edu/repos/ftouzain/vadr/vadr/0.2.0 |
| 3 | Map with minimap2 | toolshed.g2.bx.psu.edu/repos/iuc/minimap2/minimap2/2.28+galaxy1 |
| 4 | RmDup | toolshed.g2.bx.psu.edu/repos/devteam/samtools_rmdup/samtools_rmdup/2.0.1 |
| 5 | bcftools mpileup | toolshed.g2.bx.psu.edu/repos/iuc/bcftools_mpileup/bcftools_mpileup/1.15.1+galaxy3 |
| 6 | VarDict | toolshed.g2.bx.psu.edu/repos/iuc/vardict_java/vardict_java/1.8.3+galaxy1 |
| 7 | Samtools depth | toolshed.g2.bx.psu.edu/repos/iuc/samtools_depth/samtools_depth/1.15.1+galaxy2 |
| 8 | bcftools call | toolshed.g2.bx.psu.edu/repos/iuc/bcftools_call/bcftools_call/1.15.1+galaxy3 |
| 9 | vvv2_display | - png image of variants - tsv file of variants info - vcf file of variants info toolshed.g2.bx.psu.edu/repos/ftouzain/vvv2_display/vvv2_display/0.2.5.0 |
| 10 | IUPAC consensus | toolshed.g2.bx.psu.edu/repos/iuc/bcftools_consensus/bcftools_consensus/1.15.1+galaxy3 |
| 11 | majority consensus | Gives consensus with major nucleotides only toolshed.g2.bx.psu.edu/repos/iuc/bcftools_consensus/bcftools_consensus/1.15.1+galaxy3 |
Outputs
| ID | Name | Description | Type |
|---|---|---|---|
| IUPAC_consensus | #main/IUPAC_consensus | n/a |
|
| Majority_consensus | #main/Majority_consensus | n/a |
|
| significant_variants_info | #main/significant_variants_info | n/a |
|
| significant_variants_info_for_NextStrain | #main/significant_variants_info_for_NextStrain | n/a |
|
| snp_image | #main/snp_image | n/a |
|
Version History
Version 1 (earliest) Created 19th Jun 2025 at 11:27 by Fabrice Touzain
Initial commit
Open
master
f4cfc20
Creators and SubmitterCreator
Additional credit
This study was founded by the French National Research Agency and by Santé publique France as part of the project "EMERGEN". Anses Ploufragan research was also supported by Agglomération de Saint-Brieuc, Département des Côtes d'Armor and Région Bretagne
Submitter
Activity
Views: 2171 Downloads: 290 Runs: 3
Created: 19th Jun 2025 at 11:27
Last updated: 3rd Apr 2026 at 15:19
AttributionsNone
Run on Galaxy
https://orcid.org/0000-0002-1103-2425