ENA SARS-CoV-2 Nanopore Amplicon Sequencing Analysis Workflow
Version 1

Workflow Type: Nextflow
Work-in-progress

A workflow for mapping and consensus generation of SARS-CoV2 whole genome amplicon nanopore data implemented in the Nextflow framework. Reads are mapped to a reference genome using Minimap2 after trimming the amplicon primers with a fixed length at both ends of the amplicons using Cutadapt. The consensus is called using Pysam based on a majority read support threshold per position of the Minimap2 alignment and positions with less than 30x coverage are masked using ā€˜Nā€™.

Total size: 2.76 KB
help Creators and Submitter
Creators
  • David F. Nieuwenhuijse
  • Alexey Sokolov
Submitter
Activity

Views: 540   Downloads: 31

Created: 12th Feb 2021 at 11:46

Last used: 27th Jan 2022 at 05:46

help Attributions

None

Version History

Version 1 (earliest) Created 12th Feb 2021 at 11:46 by Ross Thorne

Added/updated 1 files


Open master 24e131f

Related items

Powered by
(v.1.12.0-master)
Copyright © 2008 - 2021 The University of Manchester and HITS gGmbH

By continuing to use this site you agree to the use of cookies