The Bio2 core is a scientifically complex facility that provides data analysis, scientific computing and data management support to a wide range of scientists within the Wellcome-MRC Institute of Metabolic Science (IMS) and across the wider scientific community in Cambridge.The remit of the core includes:Use, develop and maintain state of the art SOP and methods for the analysis (and visualisation) of scientific data.Provide assistance in biostatistics and experimental design.Mine and analyse the
A [Snakemake][sm] workflow for using PolyA_DB and UCSC Liftover with Cellranger.
Some genes are not accurately annotated in the reference genome.
we use information provide by the [PolyA_DB v3.2][polya] to update the coordinates,
then the [USCS Liftover][liftover] tool to update to a more recent genome.
we use [Cellranger][cr] to create the reference and count matrix.
by taking advantage of the integrated [Conda][conda] and