Assembly-Hifi-only-VGP3/main

View on GitHub Download RO-Crate Run on Galaxy
Workflow Type: Galaxy

Contiging Solo w/HiC:

Generate phased assembly based on PacBio Hifi Reads using HiC data from the same individual for phasing.

Inputs

  1. Hifi long reads [fastq]
  2. HiC forward reads (if multiple input files, concatenated in same order as reverse reads) [fastq]
  3. HiC reverse reads (if multiple input files, concatenated in same order as forward reads) [fastq]
  4. K-mer database [meryldb]
  5. Genome profile summary generated by Genomescope [txt]
  6. Name of first assembly
  7. Name of second assembly

Outputs

  1. Haplotype 1 assembly
  2. Haplotype 2 assembly
  3. QC: BUSCO report for both assemblies
  4. QC: Merqury report for both assemblies
  5. QC: Assembly statistics for both assemblies
  6. QC: Nx plot for both assemblies
  7. QC: Size plot for both assemblie

SEEK ID: https://workflowhub.eu/workflows/612?version=4

Inputs

ID Name Description Type
Bits for Hifiasm bloom filter Bits for Hifiasm bloom filter Defaults to 37 if not specified. For genomes much larger than human, applying -f38 or even -f39 is preferred to save memory on k-mer counting.
  • int?
Genomescope Summary Genomescope Summary n/a
  • File
Meryl Database Meryl Database n/a
  • File
Name of alternate assembly Name of alternate assembly n/a
  • string?
Name of primary assembly Name of primary assembly n/a
  • string?
Pacbio Reads Collection Pacbio Reads Collection n/a
  • File[]
SAK input file (Optional) SAK input file (Optional) n/a
  • File?

Steps

ID Name Description
7 Cutadapt toolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/4.7+galaxy0
8 Search in textfiles toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_grep_tool/9.3+galaxy0
9 Pick parameter value pick_value
10 MultiQC toolshed.g2.bx.psu.edu/repos/iuc/multiqc/multiqc/1.11+galaxy1
11 Replace Text toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_replace_in_line/9.3+galaxy0
12 Hifiasm toolshed.g2.bx.psu.edu/repos/bgruening/hifiasm/hifiasm/0.19.8+galaxy1
13 Convert Convert characters1
14 gfastats toolshed.g2.bx.psu.edu/repos/bgruening/gfastats/gfastats/1.3.6+galaxy0
15 gfastats toolshed.g2.bx.psu.edu/repos/bgruening/gfastats/gfastats/1.3.6+galaxy0
16 gfastats toolshed.g2.bx.psu.edu/repos/bgruening/gfastats/gfastats/1.3.6+galaxy0
17 gfastats toolshed.g2.bx.psu.edu/repos/bgruening/gfastats/gfastats/1.3.6+galaxy0
18 gfastats toolshed.g2.bx.psu.edu/repos/bgruening/gfastats/gfastats/1.3.6+galaxy0
19 gfastats toolshed.g2.bx.psu.edu/repos/bgruening/gfastats/gfastats/1.3.6+galaxy0
20 Cut Cut1
21 Busco toolshed.g2.bx.psu.edu/repos/iuc/busco/busco/5.5.0+galaxy0
22 Merqury toolshed.g2.bx.psu.edu/repos/iuc/merqury/merqury/1.3+galaxy3
23 Data Prep Primary n/a
24 Data Prep Alternate n/a
25 Estimated genome size param_value_from_file
26 Plotting Nx and Sizes n/a
27 gfastats toolshed.g2.bx.psu.edu/repos/bgruening/gfastats/gfastats/1.3.6+galaxy0
28 gfastats toolshed.g2.bx.psu.edu/repos/bgruening/gfastats/gfastats/1.3.6+galaxy0
29 Text reformatting toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_awk_tool/9.3+galaxy0
30 Text reformatting toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_awk_tool/9.3+galaxy0
31 Join toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_easyjoin_tool/9.3+galaxy0

Outputs

ID Name Description Type
cutadapt multiqc stats cutadapt multiqc stats n/a
  • File
multiqc html report multiqc html report n/a
  • File
Hifiasm Primary gfa Hifiasm Primary gfa n/a
  • File
Hifiasm Alternate gfa Hifiasm Alternate gfa n/a
  • File
Usable GFA Primary Usable GFA Primary n/a
  • File
Usable GFA Alternate Usable GFA Alternate n/a
  • File
Hifiasm Primary assembly Hifiasm Primary assembly n/a
  • File
Hifiasm Alternate assembly Hifiasm Alternate assembly n/a
  • File
Busco summary image Busco summary image n/a
  • File
Merqury png Merqury png n/a
  • File
Merqury completeness stats Merqury completeness stats n/a
  • File
Merqury Histograms Merqury Histograms n/a
  • File
Estimated Genome size Estimated Genome size n/a
  • File
Size Plot Size Plot n/a
  • File
Nx Plot Nx Plot n/a
  • File
Assembly Stats on Primary assembly Assembly Stats on Primary assembly n/a
  • File
Assembly Stats on Alternate Assembly Assembly Stats on Alternate Assembly n/a
  • File
Assembly statistics Assembly statistics n/a
  • File

Version History

v0.2.1 (latest) Created 7th Oct 2024 at 16:34 by WorkflowHub Bot

Updated to v0.2.1


Frozen v0.2.1 1262df6

v0.2 Created 3rd Aug 2024 at 03:01 by WorkflowHub Bot

Updated to v0.2


Frozen v0.2 173b906

v0.1.8 Created 24th Jul 2024 at 03:02 by WorkflowHub Bot

Updated to v0.1.8


Frozen v0.1.8 f991481

v0.1.7 Created 18th Jul 2024 at 03:02 by WorkflowHub Bot

Updated to v0.1.7


Frozen v0.1.7 3dd5731

v0.1.6 Created 10th Jul 2024 at 03:02 by WorkflowHub Bot

Updated to v0.1.6


Frozen v0.1.6 8d8c799

v0.1.5 Created 30th Apr 2024 at 03:01 by WorkflowHub Bot

Updated to v0.1.5


Frozen v0.1.5 e57076e

v0.1.4 Created 23rd Apr 2024 at 03:02 by WorkflowHub Bot

Updated to v0.1.4


Frozen v0.1.4 262adb5

v0.1.3 Created 27th Mar 2024 at 03:02 by WorkflowHub Bot

Updated to v0.1.3


Frozen v0.1.3 9bf277d

v0.1.2 Created 13th Jan 2024 at 03:01 by WorkflowHub Bot

Updated to v0.1.2


Frozen v0.1.2 acbbe2c

v0.1.1 Created 24th Nov 2023 at 03:02 by WorkflowHub Bot

Updated to v0.1.1


Frozen v0.1.1 544422d

v0.1 (earliest) Created 20th Oct 2023 at 03:01 by WorkflowHub Bot

Updated to v0.1


Frozen v0.1 1f63570
help Creators and Submitter
Creators
Not specified
Additional credit

Galaxy, VGP

Submitter
Tools
Cutadapt
MultiQC
gfastats
BUSCO
Merqury
Bandage
License
Creative Commons Attribution 4.0 International
Activity

Views: 5398   Downloads: 1342   Runs: 1

Created: 20th Oct 2023 at 03:01

Last updated: 17th Aug 2024 at 03:02

help Tags

This item has not yet been tagged.

help Attributions

None

Total size: 136 KB
Powered by
 (v.1.16.0-main)
About WorkflowHub | Acknowledgements | Credits | Terms & Conditions | Privacy Policy | Cite us | Contact us
Copyright © 2008 - 2024 The University of Manchester and HITS gGmbH