qiime2-I-import/Ia-import-multiplexed-se
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Workflow Type: Galaxy
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Tests Importing single-end multiplexed data (not demultiplexed yet)
Inputs
| ID | Name | Description | Type |
|---|---|---|---|
| Barcodes | #main/Barcodes | Barcode sequences |
|
| Metadata | #main/Metadata | A tab separated metadata file |
|
| Metadata parameter | #main/Metadata parameter | Column name of the metadata to be used for demultiplexing |
|
| Reverse complement barcodes | #main/Reverse complement barcodes | If reverse complement of barcodes is necessary for the analysis, choose Yes. |
|
| Sequences | #main/Sequences | Single-end sequences |
|
Steps
| ID | Name | Description |
|---|---|---|
| 5 | Input files | qiime2 tools input This step allows the input of the files to be analysed and then generate a qza output, necessary for the QIIME2 pipeline. toolshed.g2.bx.psu.edu/repos/q2d2/qiime2_core__tools__import/qiime2_core__tools__import/2024.10.0+dist.h3d8a7e27 |
| 6 | Metadata as artifact | Import the metadata as artifact toolshed.g2.bx.psu.edu/repos/q2d2/qiime2_core__tools__import/qiime2_core__tools__import/2024.10.0+dist.h3d8a7e27 |
| 7 | Demultiplex single-end data | qiime2 demux emp-single Demultiplex sequence data generated with the EMP protocol. toolshed.g2.bx.psu.edu/repos/q2d2/qiime2__demux__emp_single/qiime2__demux__emp_single/2024.10.0+q2galaxy.2024.10.0 |
| 8 | Summarize demultiplexed output | qiime2 demux summarize Summarize counts per sample. (Galaxy Version 2023.5.0+q2galaxy.2023.5.0.2) toolshed.g2.bx.psu.edu/repos/q2d2/qiime2__demux__summarize/qiime2__demux__summarize/2024.10.0+q2galaxy.2024.10.0 |
Outputs
| ID | Name | Description | Type |
|---|---|---|---|
| Demultiplexed single-end data | #main/Demultiplexed single-end data | n/a |
|
| Summary of the demultiplexing | #main/Summary of the demultiplexing | n/a |
|
Version History
v0.2 (earliest) Created 30th May 2024 at 11:35 by WorkflowHub Bot
Updated to v0.2
Frozen
v0.2792997b
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Views: 5792 Downloads: 22373 Runs: 0
Created: 30th May 2024 at 11:35
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