sars-cov-2-se-illumina-wgs-variant-calling/COVID-19-SE-WGS-ILLUMINA
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Run on usegalaxy.euCOVID-19: variation analysis on WGS SE data
This workflows performs single end read mapping with bowtie2 followed by sensitive variant calling across a wide range of AFs with lofreq and variant annotation with snpEff 4.5covid19.
Inputs
| ID | Name | Description | Type |
|---|---|---|---|
| Single End Collection | Single End Collection | Illumina reads with fastqsanger encoding | n/a |
| NC_045512.2 FASTA sequence of SARS-CoV-2 | NC_045512.2 FASTA sequence of SARS-CoV-2 | Fasta sequence for Severe acute respiratory syndrome coronavirus 2 isolate Wuhan-Hu-1, complete genome | n/a |
| intervals | intervals | runtime parameter for tool SnpEff eff: | n/a |
| transcripts | transcripts | runtime parameter for tool SnpEff eff: | n/a |
Steps
| ID | Name | Description |
|---|---|---|
| 0 | Single End Collection | Illumina reads with fastqsanger encoding |
| 1 | NC_045512.2 FASTA sequence of SARS-CoV-2 | Fasta sequence for Severe acute respiratory syndrome coronavirus 2 isolate Wuhan-Hu-1, complete genome |
| 2 | fastp | toolshed.g2.bx.psu.edu/repos/iuc/fastp/fastp/0.20.1+galaxy0 |
| 3 | Bowtie2 | toolshed.g2.bx.psu.edu/repos/devteam/bowtie2/bowtie2/2.3.4.2 |
| 4 | MarkDuplicates | toolshed.g2.bx.psu.edu/repos/devteam/picard/picard_MarkDuplicates/2.18.2.2 |
| 5 | MultiQC | toolshed.g2.bx.psu.edu/repos/iuc/multiqc/multiqc/1.8+galaxy1 |
| 6 | Realign reads | toolshed.g2.bx.psu.edu/repos/iuc/lofreq_viterbi/lofreq_viterbi/2.1.5+galaxy0 |
| 7 | Insert indel qualities | toolshed.g2.bx.psu.edu/repos/iuc/lofreq_indelqual/lofreq_indelqual/2.1.5+galaxy0 |
| 8 | Call variants | toolshed.g2.bx.psu.edu/repos/iuc/lofreq_call/lofreq_call/2.1.5+galaxy0 |
| 9 | Lofreq filter | toolshed.g2.bx.psu.edu/repos/iuc/lofreq_filter/lofreq_filter/2.1.5+galaxy0 |
| 10 | SnpEff eff covid19 version | toolshed.g2.bx.psu.edu/repos/iuc/snpeff_sars_cov_2/snpeff_sars_cov_2/4.5covid19 |
Outputs
| ID | Name | Description | Type |
|---|---|---|---|
| out1 | out1 | n/a | |
| report_html | report_html | n/a | |
| report_json | report_json | n/a | |
| output | output | n/a | |
| mapping_stats | mapping_stats | n/a | |
| metrics_file | metrics_file | n/a | |
| outFile | outFile | n/a | |
| stats | stats | n/a | |
| plots | plots | n/a | |
| html_report | html_report | n/a | |
| realigned | realigned | n/a | |
| output | output | n/a | |
| variants | variants | n/a | |
| outvcf | outvcf | n/a | |
| snpeff_output | snpeff_output | n/a | |
| statsFile | statsFile | n/a |
Version History
v0.1.3 (latest) Created 21st Dec 2021 at 03:01 by WorkflowHub Bot
Updated to v0.1.3
Open
masterece9c04
v0.1 (earliest) Created 12th Mar 2021 at 13:41 by WorkflowHub Bot
Added/updated 8 files
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Creators and Submitter
Views: 1207 Downloads: 36
Created: 12th Mar 2021 at 13:41
Last updated: 21st Dec 2021 at 03:01
Last used: 27th Jun 2022 at 16:50
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IWC - Intergalactic Workflow Commission
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Public web page: https://github.com/galaxyproject/iwc
The COVID-19 pandemic is the first global health crisis to occur in the age of big genomic data. Although data generation capacity is well established and sufficiently standardized, analytical capacity is not. To establish analytical capacity it is necessary to pull together global computational resources and deliver the best open source tools and analysis workflows within a ready to use, universally accessible resource. Such a resource should not be controlled by a single research group, ...
