Workflows

This workflow

• Reconstruct phylogeny (insert fragments in a reference)
• Alpha rarefaction analysis
• Taxonomic analysis

Nanopore datasets analysis - Phylogenetic Identification - antibiotic resistance genes detection and contigs building

Microbiome - QC and Contamination Filtering

Pathogens of all samples report generation and visualization

Microbiome - Taxonomy Profiling

This workflow takes a collection of BAM (output of STAR) and a gtf. It extends the input gtf using de novo annotation.

Annotation of an assembled bacterial genomes to detect genes, potential plasmids, integrons and Insertion sequence (IS) elements.

Antimicrobial resistance gene detection from assembled bacterial genomes

Importing single-end multiplexed data (not demultiplexed yet)

Use DADA2 for sequence quality control. DADA2 is a pipeline for detecting and correcting (where possible) Illumina amplicon sequence data. As implemented in the q2-dada2 plugin, this quality control process will additionally filter any phiX reads (commonly present in marker gene Illumina sequence data) that are identified in the sequencing data, and will filter chimeric sequences.