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Assembly with Hifi reads and Trio Data
Generate phased assembly based on PacBio Hifi Reads using parental Illumina data for phasing
Inputs
- Hifi long reads [fastq]
- Concatenated Illumina reads : Paternal [fastq]
- Concatenated Illumina reads : Maternal [fastq]
- K-mer database [meryldb]
- Paternal hapmer database [meryldb]
- Maternal hapmer database [meryldb]
- Genome profile summary generated by Genomescope [txt]
- Genome model parameters generated by Genomescope [tabular]
...
VGP Workflow #1
This workflow produces a Meryl database and Genomescope outputs that will be used to determine parameters for following workflows, and assess the quality of genome assemblies. Specifically, it provides information about the genomic complexity, such as the genome size and levels of heterozygosity and repeat content, as well about the data quality.
Inputs
- A collection of Hifi long reads in FASTQ format
- k-mer length
- Ploidy
Outputs
- Meryl Database of kmer counts
...
Create Meryl Database used for the estimation of assembly parameters and quality control with Merqury. Part of the VGP pipeline.
Phasing and imputation pipeline
Workflow for clinical metaproteomics database searching
Contiging Solo:
Generate assembly based on PacBio Hifi Reads.
Inputs
- Hifi long reads [fastq]
- K-mer database [meryldb]
- Genome profile summary generated by Genomescope [txt]
- Homozygous Read Coverage. Optional, use if you think the estimation from Genomescope is inacurate.
- Genomescope Model Parameters generated by Genomescope [tabular]
- Database for busco lineage (recommended: latest)
- Busco lineage (recommended: vertebrata)
- Name of first assembly
- Name of second ...
This workflow will perform taxonomic and functional annotations using Unipept and statistical analysis using MSstatsTMT.
In proteomics research, verifying detected peptides is essential for ensuring data accuracy and biological relevance. This tutorial continues from the clinical metaproteomics discovery workflow, focusing on verifying identified microbial peptides using the PepQuery tool.
From metagenomes to peptides
Type: Nextflow
Creators: Sabrina Krakau, Leon Kuchenbecker and Till Englert
Submitter: WorkflowHub Bot