Workflows

The ultimate-level complexity workflow is one among a collection of workflows designed to address tasks up to CTF estimation. In addition to the functionalities provided by layer 0 and 1 workflows, this workflow aims to enhance the quality of both acquisition images and processing.

Quality control protocols

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Combination of methods

• CTF consensus
• New methods to compare ctf estimations
• CTF xmipp criteria (richer parameters i.e. ice detection)

Advantages: 

• Control of ...

The second-level complexity workflow is one among a collection of workflows designed to address tasks up to CTF estimation. In addition to the functionalities provided by the layer 0 workflow, this workflow aims to enhance the quality of acquisition images using quality protocols.

Quality control protocols

• Movie max shift: automatic reject those movies whose frames move more than a given threshold. 

• Tilt analysis: quality score based in the Power Spectrum Density (astigmatism and ...

The simplest workflow among a collection of workflows intended to solve tasks up to CTF estimation.

Basic processing pipeline

• Import movies

• Movie alignment (recommended 2 GPUs)

• CTF estimation

Monitor acquisition

• Monitor summary protocol: 
• Monitor basic parameters (item counts, drift, resolution, astigmatism, defocus, etc.)
• Raise alarms (mail setting available)
• Provides information of the basic steps (html report)