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MGnify genomes catalogue pipeline

MGnify A pipeline to perform taxonomic and functional annotation and to generate a catalogue from a set of isolate and/or metagenome-assembled genomes (MAGs) using the workflow described in the following publication:

Gurbich TA, Almeida A, Beracochea M, Burdett T, Burgin J, Cochrane G, Raj S, Richardson L, Rogers AB, Sakharova E, Salazar GA and Finn RD. (2023) [MGnify Genomes: A Resource for Biome-specific Microbial Genome ...



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This repository contains two workflows for metagenome and ...

Type: Common Workflow Language

Creators: None

Submitters: Germana Baldi, Varsha Kale



Pipeline to recover eukaryotic MAGs using CONCOCT, metaBAT2 and EukCC's merging algorythm.

Needs paired end shotgun metagenomic reads.


Eukrecover requires an environment with snakemake and metaWRAP.


Define your samples in the file samples.csv. This file needs to have the columns project and run to identify each metagenome.

This pipeline does not support co-binning, but feel free to change it.

Clone this repro wherever you want to run the pipeline:


Type: Snakemake

Creators: None

Submitter: Varsha Kale

Assembly and quantification metatranscriptome using metagenome data.

Version: see VERSION


MetaGT is a bioinformatics analysis pipeline used for improving and quantification metatranscriptome assembly using metagenome data. The pipeline supports Illumina sequencing data and complete metagenome and metatranscriptome assemblies. The pipeline involves the alignment of metatranscriprome assembly to the metagenome assembly with further extracting CDSs, which are covered by ...

Type: Nextflow

Creators: Andrey Prjibelski, Varsha Kale, Anton Korobeynikov

Submitter: Varsha Kale



Module for integrative Mobilome prediction

Bacteria can acquire genetic material through horizontal gene transfer, allowing them to rapidly adapt to changing environmental conditions. These mobile genetic elements can be classified into three main categories: plasmids, phages, and integrons. Autonomous elements are those capable of excising themselves from the chromosome, reintegrating elsewhere, and potentially modifying the host's physiology. Small integrative elements like insertion ...

Type: Nextflow

Creators: Alejandra Escobar, Martin Beracochea

Submitter: Alejandra Escobar


MGnify ( provides a free to use platform for the assembly, analysis and archiving of microbiome data derived from sequencing microbial populations that are present in particular environments. Over the past 2 years, MGnify (formerly EBI Metagenomics) has more than doubled the number of publicly available analysed datasets held within the resource. Recently, an updated approach to data analysis has been unveiled (version 5.0), replacing the previous single pipeline ...

Type: Common Workflow Language

Creator: Alex L Mitchell, Alexandre Almeida, Martin Beracochea, Miguel Boland, Josephine Burgin, Guy Cochrane, Michael R Crusoe, Varsha Kale, Simon C Potter, Lorna J Richardson, Ekaterina Sakharova, Maxim Scheremetjew, Anton Korobeynikov, Alex Shlemov, Olga Kunyavskaya, Alla Lapidus, Robert D Finn

Submitter: Martin Beracochea



VIRify is a recently developed pipeline for the detection, annotation, and taxonomic classification of viral contigs in metagenomic and metatranscriptomic assemblies. The pipeline is part of the repertoire of analysis services offered by MGnify. VIRify’s taxonomic classification relies on the detection of taxon-specific profile hidden Markov models (HMMs), built upon a set of 22,014 orthologous protein domains and referred to as ViPhOGs. VIRify was implemented in CWL. What do I need? The ...

Type: Nextflow

Creators: Martin Beracochea, Martin Hölzer, Alexandre Almeida, Guillermo Rangel-Pineros and Ekaterina Sakharova

Submitter: Laura Rodriguez-Navas

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