Sanger Tree of Life Assembly method

The Tree of Life Programme uses DNA sequencing and cellular technologies to investigate the diversity and origins of life on Earth. Our research covers all eukaryotic organisms, which means all complex life with a nucleus in their cells – that is every animal, plant, fungi and protist on the planet.

We use Nextflow and are building our own pipelines following the nf-core methodology. All our pipelines are on GitHub, https://github.com/sanger-tol, and are then listed and documented at ...

We gather the high throughput sequence data as it is generated, run quality control analysis to make sure the data is good quality and from the expected species. We assemble the long read sequence data into long contiguous pieces, then use further long-range sequencing data to scaffold the data into chromosome scale blocks. We then manually curate the assembly and submit it to INSDC.

We also develop automated tools and pipelines to enable the high throughput required for ever growing number of ...