Biomarker screening in preeclampsia
Version 1

Workflow Type: Galaxy

Objective. Biomarkers have become important for the prognosis and diagnosis of various diseases. High-throughput methods such as RNA-sequencing facilitate the detection of differentially expressed genes (DEGs), hence potential biomarker candidates. Individual studies suggest long lists of DEGs, hampering the identification of clinically relevant ones. Concerning preeclampsia, a major obstetric burden with high risk for adverse maternal and/or neonatal outcomes, limitations in diagnosis and prediction are still important issues. Therefore, we developed a workflow to facilitate the screening for biomarkers. Methods. Based on the tool DeSeq2, we established a comprehensive workflow for the identification of DEGs, analyzing data from multiple publicly available RNA-sSequencing studies. We applied it to four RNA-sSequencing datasets (one blood, three placenta) analyzing patients with preeclampsia and normotensive controls. We compared our results with other published approaches and evaluated their performance. Results. We identified 110 genes dysregulated in preeclampsia, observed in ≥3 of the analyzed studies, six even in all four studies. Among them were FLT-1, TREM-1, and FN1 which either represent established biomarkers on protein level, or promising candidates based on recent studies. In comparison, using a published meta-analysis approach we obtained 5,240 DEGs. Conclusions. We present a data analysis workflow for preeclampsia biomarker screening, capable of identifying significant biomarker candidates, while drastically decreasing the numbers of candidates. Moreover, we were also able to confirm its performance for heart failure. Our approach can be applied to additional diseases for biomarker identification and the set of identified DEGs in preeclampsia represents a resource for further studies.

Inputs

ID Name Description Type
library library runtime parameter for tool Bowtie2 n/a
library library runtime parameter for tool Bowtie2 n/a
adapters adapters runtime parameter for tool FastQC n/a
contaminants contaminants runtime parameter for tool FastQC n/a
input_file input_file runtime parameter for tool FastQC n/a
limits limits runtime parameter for tool FastQC n/a
alignment alignment runtime parameter for tool featureCounts n/a
alignment alignment runtime parameter for tool featureCounts n/a
batch_factors batch_factors runtime parameter for tool DESeq2 n/a
batch_factors batch_factors runtime parameter for tool DESeq2 n/a

Steps

ID Name Description
0 Download and Extract Reads in FASTA/Q toolshed.g2.bx.psu.edu/repos/iuc/sra_tools/fastq_dump/2.11.0+galaxy0
1 Bowtie2 toolshed.g2.bx.psu.edu/repos/devteam/bowtie2/bowtie2/2.4.2+galaxy0
2 FastQC toolshed.g2.bx.psu.edu/repos/devteam/fastqc/fastqc/0.73+galaxy0
3 featureCounts toolshed.g2.bx.psu.edu/repos/iuc/featurecounts/featurecounts/2.0.1+galaxy2
4 featureCounts toolshed.g2.bx.psu.edu/repos/iuc/featurecounts/featurecounts/2.0.1+galaxy2
5 DESeq2 toolshed.g2.bx.psu.edu/repos/iuc/deseq2/deseq2/2.11.40.7+galaxy1
6 DESeq2 toolshed.g2.bx.psu.edu/repos/iuc/deseq2/deseq2/2.11.40.7+galaxy1

Outputs

ID Name Description Type
output_accession output_accession n/a
output output n/a
html_file html_file n/a
text_file text_file n/a
output_short output_short n/a
output_summary output_summary n/a
output_short output_short n/a
output_summary output_summary n/a
deseq_out deseq_out n/a
plots plots n/a
deseq_out deseq_out n/a
plots plots n/a

Version History

Version 1 (earliest) Created 3rd May 2022 at 14:05 by Marlene Rezk

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Frozen Version-1 df4a826
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Citation
Rezk, M. (2022). Biomarker screening in preeclampsia. WorkflowHub. https://doi.org/10.48546/WORKFLOWHUB.WORKFLOW.338.1
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Created: 3rd May 2022 at 14:05

Last used: 27th May 2022 at 23:47

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