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JAX NGS Operations Nextflow DSL2 Pipelines
Stable

DOI

JAX NGS Operations Nextflow DSL2 Pipelines

This repository contains production bioinformatic analysis pipelines for a variety of bulk 'omics data analysis. Please see the Wiki documentation associated with this repository for all documentation and available analysis workflows.

Type: Nextflow

Creators: Michael Lloyd, Brian Sanderson, Barry Guglielmo, Sai Lek, Peter Fields, Harshpreet Chandok, Carolyn Paisie, Gabriel Rech, Ardian Ferraj, Anuj Srivastava

Submitter: Michael Lloyd

DOI: 10.48546/workflowhub.workflow.874.1

Tango: Numerical reconciliation of bacterial fermentation in cheese production
Stable

Complete workflow for TANGO as reported in Lecomte et al (2024), "Revealing the dynamics and mechanisms of bacterial interactions in cheese production with metabolic modelling", Metabolic Eng. 83:24-38 https://doi.org/10.1016/j.ymben.2024.02.014

  1. Parameters for individual models are obtained by optimization
  2. Individual dynamics and community dynamics are simulated
  3. Figures for the manuscript are assembled from the results.

Type: Common Workflow Language

Creators: None

Submitter: David James Sherman

Assembly-Hifi-Trio-phasing-VGP5/main

Assembly with Hifi reads and Trio Data

Generate phased assembly based on PacBio Hifi Reads using parental Illumina data for phasing

Inputs

  1. Hifi long reads [fastq]
  2. Concatenated Illumina reads : Paternal [fastq]
  3. Concatenated Illumina reads : Maternal [fastq]
  4. K-mer database [meryldb]
  5. Paternal hapmer database [meryldb]
  6. Maternal hapmer database [meryldb]
  7. Genome profile summary generated by Genomescope [txt]
  8. Genome model parameters generated by Genomescope [tabular]

...

Type: Galaxy

Creator: Galaxy, VGP

Submitter: WorkflowHub Bot

qiime2-I-import/QIIME2-Ia import multiplexed data single end

Importing single-end multiplexed data (not demultiplexed yet)

Type: Galaxy

Creators: Debjyoti Ghosh, Helmholtz-Zentrum für Umweltforschung - UFZ

Submitter: WorkflowHub Bot

qiime2-II-denoising/QIIME2-IIa denoising and feature table creation for single ended data

Use DADA2 for sequence quality control. DADA2 is a pipeline for detecting and correcting (where possible) Illumina amplicon sequence data. As implemented in the q2-dada2 plugin, this quality control process will additionally filter any phiX reads (commonly present in marker gene Illumina sequence data) that are identified in the sequencing data, and will filter chimeric sequences.

Type: Galaxy

Creators: Debjyoti Ghosh, Helmholtz-Zentrum für Umweltforschung - UFZ

Submitter: WorkflowHub Bot

consensus-peaks/consensus-peaks-chip-sr

This workflow takes as input SR BAM from ChIP-seq. It calls peaks on each replicate and intersect them. In parallel, each BAM is subsetted to smallest number of reads. Peaks are called using all subsets combined. Only peaks called using a combination of all subsets which have summits intersecting the intersection of at least x replicates will be kept.

Type: Galaxy

Creator: Lucille Delisle

Submitter: WorkflowHub Bot

polish-with-long-reads/main

Racon polish with long reads, x4

Type: Galaxy

Creator: Anna Syme

Submitter: WorkflowHub Bot

repeatmasking/main

RepeatMasking Workflow

This workflow uses RepeatModeler and RepeatMasker for genome analysis.

  • RepeatModeler is a software package for identifying and modeling de novo families of transposable elements (TEs). At the heart of RepeatModeler are three de novo repeat search programs (RECON, RepeatScout and LtrHarvest/Ltr_retriever) which use complementary computational methods to identify repeat element boundaries and family relationships from sequence data.

  • RepeatMasker is a program that analyzes ...

Type: Galaxy

Creator: Romane Libouban

Submitter: WorkflowHub Bot

average-bigwig-between-replicates/main

We assume the identifiers of the input list are like: sample_name_replicateID. The identifiers of the output list will be: sample_name

Type: Galaxy

Creator: Lucille Delisle

Submitter: WorkflowHub Bot

sars-cov-2-pe-illumina-artic-variant-calling/COVID-19-PE-ARTIC-ILLUMINA

The workflow for Illumina-sequenced ARTIC data builds on the RNASeq workflow for paired-end data using the same steps for mapping and variant calling, but adds extra logic for trimming ARTIC primer sequences off reads with the ivar package. In addition, this workflow uses ivar also to identify amplicons affected by ARTIC primer-binding site mutations and tries to exclude reads derived from such tainted amplicons when calculating allele-frequencies of other variants.

Type: Galaxy

Creator: Wolfgang Maier

Submitter: WorkflowHub Bot

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