The second-level complexity workflow is one among a collection of workflows designed to address tasks up to CTF estimation. In addition to the functionalities provided by the layer 0 workflow, this workflow aims to enhance the quality of acquisition images using quality protocols. **Quality control protocols** * **Movie max shift**: automatic reject those movies whose frames move more than a given threshold. * **Tilt analysis**: quality score based in the Power Spectrum Density (astigmatism and tilt) * **CTF consensus**: acts as a filter discarding micrographs based on their CTF (limit resolution, defocus, astigmatism, etc.). **Advantages:** * More control of the acquisition quality * Reduce unnecessary processing time and storage