This workflow performs colour deconvolution and automated thresholding to quantify stained area in brightfield histological images (MT, IHC). It accepts whole slides or pre-cropped ROIs. ## Associated Tutorial This workflows is part of the tutorial [Quantitative Analysis of Histological Staining Using Color Deconvolution](https://training.galaxyproject.org/training-material/topics/imaging/tutorials/stain-quantification-color-deconvolution/tutorial.html), available in the [GTN](https://training.galaxyproject.org) ## Features * Includes [Galaxy Workflow Tests](https://training.galaxyproject.org/training-material/faqs/gtn/workflow_run_test.html) * Includes a [Galaxy Workflow Report](https://training.galaxyproject.org/training-material/faqs/galaxy/workflows_report_view.html) * Uses [Galaxy Workflow Comments](https://training.galaxyproject.org/training-material/faqs/galaxy/workflows_comments.html) ## Thanks to... **Workflow Author(s)**: Diana Chiang Jurado **Tutorial Author(s)**: [Diana Chiang Jurado](https://training.galaxyproject.org/training-material/hall-of-fame/dianichj/) **Tutorial Contributor(s)**: [Leonid Kostrykin](https://training.galaxyproject.org/training-material/hall-of-fame/kostrykin/) **Funder(s)**: [University of Freiburg](https://training.galaxyproject.org/training-material/hall-of-fame/uni-freiburg/) **Grants(s)**: [NFDI4Bioimage](https://training.galaxyproject.org/training-material/hall-of-fame/nfdi4bioimage/) [](https://training.galaxyproject.org/training-material/)