The present workflow allows to perform 2D spots/blobs detection on specific channel and stack of an image. Images will be directly fetched from IDR by including the IDs. A pre-processing steps with histogram normalization is performed before the blobs detection. ## Associated Tutorial This workflows is part of the tutorial [Quantification of single-molecule RNA fluorescence in situ hybridization (smFISH) in yeast cell lines](https://training.galaxyproject.org/training-material/topics/imaging/tutorials/2D-spot-detection/tutorial.html), available in the [GTN](https://training.galaxyproject.org) ## Features * Includes [Galaxy Workflow Tests](https://training.galaxyproject.org/training-material/faqs/gtn/workflow_run_test.html) * Includes a [Galaxy Workflow Report](https://training.galaxyproject.org/training-material/faqs/galaxy/workflows_report_view.html) * Uses [Galaxy Workflow Comments](https://training.galaxyproject.org/training-material/faqs/galaxy/workflows_comments.html) ## Thanks to... **Workflow Author(s)**: Riccardo **Tutorial Author(s)**: [Riccardo Massei](https://training.galaxyproject.org/training-material/hall-of-fame/rmassei/) **Tutorial Contributor(s)**: [Leonid Kostrykin](https://training.galaxyproject.org/training-material/hall-of-fame/kostrykin/), [Riccardo Massei](https://training.galaxyproject.org/training-material/hall-of-fame/rmassei/), [Björn Grüning](https://training.galaxyproject.org/training-material/hall-of-fame/bgruening/) **Funder(s)**: [Deutsche Forschungsgemeinschaft](https://training.galaxyproject.org/training-material/hall-of-fame/dfg/) **Grants(s)**: [NFDI4Bioimage](https://training.galaxyproject.org/training-material/hall-of-fame/nfdi4bioimage/) [](https://training.galaxyproject.org/training-material/)